Unit-I
Introduction to Genomics, DNA sequencing methods manual & automated: Maxam & Gilbert and Sangers method. Pyrosequencing, Genome Sequencing: Shotgun & Hierarchical (clone contig) methods, Computer tools for sequencing projects: Genome sequence assembly software.
Unit-II
Managing and Distributing Genome Data: Web-based servers and software for genome analysis: ENSEMBL, VISTA, UCSC Genome Browser, NCBI genome. Selected Model Organisms Genomes and Databases.
Unit-III
Introduction to protein structure, Chemical properties of proteins. Physical interactions that determine the property of proteins. Short-range interactions, electrostatic forces, van der Waals interactions, hydrogen bonds, Hydrophobic interactions.
Determination of sizes (Sedimentation analysis, gel filtration, SDS-PAGE); Native PAGE, Determination of covalent structures, Edman degradation.
Unit-IV
Introduction to Proteomics, Analysis of proteomes. 2D-PAGE. Sample preparation, solubilization, reduction, resolution. Reproducibility of 2D-PAGE. Mass spectrometry-based methods for protein identification. De novo sequencing using mass spectrometric data.