Unit-I
History of Bioinformatics. The notion of Homology. Sequence Information Sources, EMBL, GENBANK, Entrez, Unigene, Understanding the structure of each source and using it on the web.
Unit-II
Protein Information Sources, PDB, SWISSPROT, TREMBL, Understanding the structure of each source and using it on the web. Introduction of Data Generating Techniques and Bioinformatics problems posed by them-Restriction Digestion, Chromatograms, Blots, PCR, Mass Spectrometry.
Unit-III
Sequence and Phylogeny analysis, Detecting Open Reading Frames, Introduction to BLAST, using it on the web, Outline of sequence Assembly, Pairwise Alignments, Interpreting results, Multiple Sequence Alignment, Phylogenetic Analysis.
Unit-IV
Searching Databases: SRS, Entrez, Sequence Similarity Searches-BLAST, FASTA, Data Submission. Genome Annotation: Pattern and repeat finding, Gene identification tools.